FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l04n02_159.3520000006b295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n02_159.3520000006b295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31194306
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG191600.018.502795
CTATACT197500.017.8780944
GTATAGG143050.017.3834631
TACACTG148550.017.1789075
TAGACTG101950.016.7340535
CCTATAC140200.016.3726963
GTATTGG234900.015.7477691
TATACAG178550.015.4635865
ACTGTGC141500.015.3419898
TCTATAC151900.015.3306093
GTATATA399000.014.8003231
GTGTAGG135950.014.7939311
CTACACT145700.014.710134
ATACTGT204900.014.6991956
TTATACT234250.014.5458484
TACACAG139050.014.4223285
TAATACT230400.014.3970154
ACAGTGC151700.014.3104258
TAGACAG109650.014.2154015
ACTGTCC100050.014.1488488