FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l04n02_154.3520000006b21e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n02_154.3520000006b21e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19624700
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG102100.021.420841
CCTATAC107700.020.65133
CTATACT143600.020.4518434
GTATTGG136300.017.6505831
TATACTG115100.016.9692525
TAGACTG66800.016.5758465
TACACTG93200.016.4186025
CCCTATA103750.016.4022732
GTGTAGG77400.016.2168831
TATAGGG98750.015.9812282
TATACAG108350.015.7895515
TATACTA191700.015.5680325
ACTGTGC93000.015.5277938
TATTGGG146850.014.9546812
GTATATA254150.014.6853391
TCTATAC95550.014.3742233
TACACCG62750.014.3134485
TACACAG82750.014.2997215
TGCGTTC101150.013.9936769
TAGACAG63850.013.8435755