FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l04n02_153.3520000006b196.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n02_153.3520000006b196.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31281726
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG186600.017.8769975
TAGACTG102850.017.0948965
GTATAGG135650.016.7175751
GTATTGG258300.016.4730241
TACACTG146550.016.3747485
CTACACT147300.015.9367414
ACTGTGC136800.015.6950398
CTATACT191200.015.4588744
TACACCG110150.015.4442775
TATACAG180750.014.69611555
CCTATAC135700.014.6382483
TAGACAG111550.014.568865
GTATATA406650.014.5670631
GTGTAGG135450.014.5310181
TCTATAC157650.014.0470343
TACACAG138650.014.0175635
TATTGGG273350.013.9281352
GTATAGA157250.013.7561041
GTATAAG143100.013.6877571
CCTACAC133100.013.6388583
Produced by FastQC (version 0.11.2)