FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l04n02_148.3520000006b0f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n02_148.3520000006b0f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19703030
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG98100.017.9328731
TAGACTG62800.017.6311995
TATACTG111750.016.9247045
GTGTAGG86150.016.2259161
ACTGTGC89500.016.1350658
TACACTG88850.015.7244495
TACACCG75250.015.47195155
CCTATAC98450.015.01258853
TAGGGGT156400.014.8278254
TAGACAG67150.014.5075265
GGACTGT61900.014.4280716
CTACACC112200.014.4005944
GTCCTAG72300.014.0731941
TGGACTG94350.014.0523145
GTCCTGG88850.014.020421
CTATACT134200.013.9874634
ACTGCGC54850.013.9434228
GTCCAGG93350.013.7520241
TGTACTG130450.013.6606925
CTAGGGG115850.013.6602633