FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l04n02_146.3520000006b08a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n02_146.3520000006b08a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36540159
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG206450.020.9809511
CTATACT281200.020.6518924
TATACTG223800.020.1724745
CCTATAC220400.019.148043
TAGACTG126600.018.8061395
TACACTG174400.017.2485625
ACTGTGC168250.016.7425068
TACACAG155700.016.573225
CTACACT169250.016.5663684
GTATTGG230500.016.316511
TATAGGG201550.016.0848642
CCTACAC151800.015.8103913
ATACTGT233750.015.648816
CTAGACT148450.015.4941994
TTATACT308250.015.4941214
TATACAG212150.015.2545615
TCTATAC191300.015.1792133
CCCTATA206250.015.0269012
TAATACT309500.015.0169654
ACAGTGC181100.015.0037028