FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l04n01_162.3510000006b303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n01_162.3510000006b303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20098136
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG125450.024.8510591
CCTATAC134400.021.205773
CTATACT161800.020.9907254
TATAGGG121450.019.986282
TATACTG118750.019.7197485
TAGACTG68800.018.778835
CCCTATA128400.017.6096422
TATACTA214700.016.614835
TACACTG94200.016.085255
TATACAG116600.015.9689225
ACTGTGC92350.015.892828
GTATTGG123550.015.6938381
TACACCG69850.015.4364525
TAGGGAT133950.015.42575554
TAATACT145600.015.1375834
TAGACAG65400.014.5258065
CTACACT92750.014.4934724
ATAGGGA132750.014.4918093
GTATATA262300.014.38579751
TACACAG85850.014.3300445