FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l04n01_159.3510000006b298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n01_159.3510000006b298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31194306
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG185950.018.4173155
GTATAGG143900.017.701691
CTATACT196650.017.4881974
TACACTG147200.017.2636725
CCTATAC144050.016.0919653
TATACAG184050.015.9492375
TAGACTG102800.015.7560915
TACACCG111250.015.6694325
ACTGTCC102950.015.3637828
GTATTGG235650.015.0849851
TCTATAC150000.014.8519723
ACTGTGC144350.014.7743988
CCTACAC130550.014.6996523
CTACACT149600.014.5740944
TATACCG103100.014.5584675
GTATAGA164150.014.2440981
GTATTAG142000.014.2236591
TAATACT234000.014.1690264
TTATACT240650.014.0538274
CCTAGAC97400.014.0454633