FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l04n01_153.3510000006b199.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n01_153.3510000006b199.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31281726
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG190500.018.9497725
TACACTG148200.017.3394135
CTATACT193700.017.3374084
GTATTGG258800.017.2230741
GTATAGG135950.016.2884141
TAGACTG102250.015.9336815
TACACAG139700.015.60635955
TACACCG104800.015.5459815
TATACCG97600.015.4274715
ACTGTGC137300.015.2216328
CTACACT149450.015.00169854
TATACAG173000.014.9910435
CCTACAC132450.014.7755063
CCTATAC130600.014.6574683
TCTATAC158000.014.5206873
TATTGGG274200.014.413162
GTATAGA165650.014.1999181
TAGACAG107950.014.1243255
GTCTAGG96300.014.1127561
GTATATA404450.014.0990071
Produced by FastQC (version 0.11.2)