FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l04n01_148.3510000006b0f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n01_148.3510000006b0f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19703030
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG60700.018.2329395
GTATAGG99900.017.5536081
ACTGTGC90750.017.324648
ACAGTGC98200.017.2195328
CCTATAC104650.016.7036023
TAGACAG65200.016.246015
CTATACT135700.016.0319424
TATACTG109500.015.9633175
GTGTAGG82950.015.9269971
TACACTG91650.015.5999595
TACACCG76000.015.4998525
TACACAG85400.015.0730425
CTACACC109850.015.0480314
CCTACAC91300.014.5676273
GTCCTAG73300.014.5227741
AGACAGT76650.014.5006186
GGACCGT53400.014.4987046
CTAGGAC76750.014.420463
TAGGACT81300.014.3729214
ACTGCGC50000.014.3446448
Produced by FastQC (version 0.11.2)