FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l04n01_145.3510000006b057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n01_145.3510000006b057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24648700
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG162150.022.8592221
CTATACT208400.021.8126134
CCTATAC169350.021.1766343
TATACTG151250.019.7218675
TATAGGG152350.019.174942
TAGACTG82300.018.3531675
CCCTATA157550.018.2707162
TACACTG115300.017.3022965
TATACTA282100.017.0901835
GTATTGG173250.016.4299931
ACTGTGC116350.016.2069998
TACACCG84250.015.842375
TATACCG80200.015.5171045
TCTATAC125250.015.4351863
TATACAG150900.015.4237725
TACACAG108900.015.309575
TAATACT178400.015.2830654
GTATAGA122600.015.1942541
TTATACT190100.015.1670114
TAGGGAT171350.015.16340354