FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l03n02_127.3520000006b3a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l03n02_127.3520000006b3a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27922259
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG174700.019.4426165
TAGACTG89600.018.821835
TACACTG128300.018.5873975
GTATAGG135150.018.4971891
ACTGTGC128750.017.8559138
CTATACT206050.017.8217664
CCTATAC144900.016.8843173
CTACACT130250.016.6314834
CCAGGAC107050.016.419493
ATACTGT179350.016.288736
TGGACTG129550.016.1712175
TAGACAG94550.015.8267075
TTACACT152300.015.7207954
CTACCCT108400.015.6890844
GTATTGG196700.015.4636371
TACACAG124150.015.4588145
ACTGTCC86900.015.4139278
GGACTGT85100.015.3502926
ACAGTGC142050.015.2812528
ACTGTGT136500.015.1718248
Produced by FastQC (version 0.11.2)