FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l03n02_126.3520000006b344.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l03n02_126.3520000006b344.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20778538
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG114200.021.765911
TATACTG130450.020.7572695
CTATACT185600.020.348424
CCTATAC132200.019.260873
TACACTG91700.019.0123655
ACTGTGC94200.018.1524248
TAGACTG66500.017.2875395
TATACCG61850.017.127925
GTGTAGG79300.017.0809841
CTACACT96500.016.983744
GTATTGG144900.016.3343621
ACAGTGC104950.016.0215248
TTATACT198950.015.9504914
GTATAGA129000.015.6949931
TATAGGG116750.015.5077452
ATACTGT135750.015.361586
TAGACAG73400.015.3388125
TACACAG92050.015.3275285
CCCTATA129850.015.2973052
CTGTGCG51800.015.221499