FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l03n02_124.3520000006b2cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l03n02_124.3520000006b2cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23127009
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG160050.024.4382131
CTATACT219400.021.0458354
TATACTG160100.020.6219185
TATAGGG154000.020.4577542
CCTATAC173400.019.8074053
TAATACT207750.019.230544
ATACTGT153700.017.7087616
TTATACT211900.017.3967154
TAGACTG82600.017.3685075
TATACTA298350.017.2280775
CCCTATA167800.016.7929882
ACTGTGC112600.016.7892558
TATACAG151750.016.5913895
GTATATA368700.016.4347231
GTATAAT193750.016.2383381
GTATTAT190000.015.9084851
GTATTAG115300.015.8280431
ATTATAC181850.015.6216283
TAGGGAT165200.015.29812054
TACACTG119200.015.2636395