FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l03n02_121.3520000006b146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l03n02_121.3520000006b146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21472806
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG152800.026.6547741
CTATACT201050.023.462954
CCTATAC170450.022.1568283
TATACTG139450.021.4273555
TATAGGG147850.021.3410472
CCCTATA160600.019.321312
TATACTA265800.019.1055325
TTATACT180950.018.5346154
TAATACT180750.018.2660224
GTATTAT162550.018.0098971
TATACAG137400.017.5635935
TAGACTG76950.017.4091245
GTATATA311900.017.248391
ATACTGT135000.016.9247976
ACTGTGC105000.016.692528
TAGGGAT160650.016.6776874
GTATTAG106700.016.613531
ATAGGGA153100.016.6003043
GTATAAG102800.016.596591
TCTATAC114550.016.4224343