FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l03n01_128.3510000006b42c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l03n01_128.3510000006b42c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26402696
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT213100.021.9483454
CCTATAC153400.021.7268733
TATACTG167700.020.7341525
GTATAGG140000.020.1719441
TAGACTG83300.018.3506685
TACACTG118650.017.7742655
GTGTAGG100100.017.6887971
CTACACT117950.017.5712474
ACTGTGC119000.017.0876458
CCCTATA146100.016.6781392
ATACTGT170350.016.3957566
CTAGACT95700.016.1545894
GGACCGT57750.015.7672956
ACTGTCC77900.015.6860728
ACAGTGC137200.015.6462098
GTATTGG169800.015.6087721
TCTATAC150000.015.4975283
TAGACAG85850.015.4974665
GACTGTG73150.015.4792097
GGACTGT76200.015.4168726