FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l03n01_127.3510000006b3a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l03n01_127.3510000006b3a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27922259
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG129350.019.3310245
GTATAGG126850.019.2683411
TATACTG168650.019.1344155
TAGACTG88350.018.9568965
CTATACT197950.018.713014
CCTATAC137450.017.6132493
CTACACT126500.017.4543384
ACTGTGC126750.016.6009258
ACAGTGC141250.016.4665998
CCTACAC110150.016.331313
TAGGACT111500.015.7313944
ATACTGT175550.015.6679846
GTATAGA160500.015.5429431
CTAGACT104300.015.5278474
GTGTAAG91450.015.3864461
TAGACAG94500.015.3767425
ACTGTGT135450.015.3256568
GTATTGG188400.015.3240861
CAGAGGT169100.015.2775964
GACTGTG84800.015.2440427
Produced by FastQC (version 0.11.2)