FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l03n01_124.3510000006b2ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l03n01_124.3510000006b2ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23127009
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG160600.025.2837311
CTATACT210300.023.6611064
TATAGGG152550.021.9169982
TATACTG157900.021.8731585
CCTATAC166700.021.230393
TAATACT197200.019.8744644
TAGACTG84400.018.2245855
TTATACT204250.017.8745614
TATACTA303050.017.7333985
CCCTATA161750.017.7323042
ACTGTGC117050.017.6154028
ATAATAC171250.017.3361953
GTATTAT173800.017.2490481
ATACTGT158850.016.989926
GTATATA359550.016.52021
GTATAAG114550.016.5033341
ATAGGGA159500.016.2997953
GTATTAG115000.016.292851
TAGGGAT167650.016.2408854
TCTATAC124250.016.1542913