FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n02_95.3520000006b1f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n02_95.3520000006b1f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13355448
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG72600.015.3128945
GTATAGG53550.014.7333771
CTATACT78850.014.5813664
TAGACAG44150.014.31195455
TAGACTG44300.014.1562515
ACTGTGC57250.014.1043768
CGCCGTA68150.013.45138694-95
GTATTGG107500.013.2637561
GTCCTAG46350.013.2279841
CTACACT60350.013.1469124
TCTATAC61600.013.0352153
GTATAGA61050.013.0012321
GTGTAGG57500.012.8946771
GTCTTAG39150.012.8684961
GTATATA158900.012.8616971
CCTAGAC40750.012.8255883
CCTATAC55750.012.6985333
TACACTG58850.012.5937815
GACTGTG38950.012.561167
TACACAG55250.012.5544775