FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n02_87.3520000006b102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n02_87.3520000006b102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46862694
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG295600.017.952425
TAGACTG153700.016.9387345
CTATACT339950.015.9738494
TAATACT447050.015.93034
TACACTG218350.015.7746255
TTATACT438150.015.4948654
CTAGACT175400.015.4662464
ACTGTGC218050.015.4226568
TAGACAG155200.015.2444525
ATACTGT311500.014.9748766
TTACACT260050.014.7067754
CTGTGCA312500.014.4394449
TAAGGCT190850.014.4382394
TAAGACT211400.014.3157094
GTATTGG313500.014.1455111
GTATAGA280000.014.1234181
GTATAGG226150.014.0606061
CCAGGAC176800.014.0283173
CTACACT222250.013.8305974
TCTATAC263600.013.7890193
Produced by FastQC (version 0.11.2)