FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n02_86.3520000006b0b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n02_86.3520000006b0b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36132769
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG218750.017.548475
CTATACT240300.014.9665264
TAATACT312350.014.9517124
TTATACT304300.014.894484
TACACTG164900.014.463025
TAGACTG120750.013.9281045
ACTGTGC158550.013.8107368
CTACACT165050.013.3561534
GTATAGG142800.013.3472651
ATAATAC276250.013.2780293
GTATTGG296200.013.2547381
TAGACAG122100.013.1515495
TATACAG209800.013.0887535
GTATAGA201600.013.0144161
ATACTGT227600.013.0023546
TAAGACT154550.012.8802394
TTACACT188750.012.7614474
CTAGACT133250.012.6929244
CTGTGCG91800.012.6248179
TAAGGCT140500.012.4437254