FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n02_102.3520000006b36d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n02_102.3520000006b36d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30556443
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG178200.020.6442551
CTATACT225150.020.0249354
TATACTG168300.018.772015
CCTATAC189400.018.388053
TATAGGG172150.015.9267582
TATACTA289950.015.680585
TTATACT214300.015.4077584
TAGACTG98450.015.2973715
ACTGTGC137300.014.9104658
CCCTATA183600.014.7264542
TCTATAC147900.014.6811373
TATACAG166200.014.5784865
TAATACT212700.014.5408684
TACACTG133050.014.49721055
GTATTAG130350.013.7101681
TAGGACT121600.013.6744974
GTATTAT198500.013.6483551
GTATATA356900.013.4772081
ATTATAC191550.013.444013
GTATAAG134050.013.4381141