FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n02_101.3520000006b2e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n02_101.3520000006b2e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57245972
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG301950.017.3860245
CTATACT323350.016.3094
GTATAGG247400.016.2532671
TAATACT360050.015.4647194
GTATTGG418750.015.1529251
TATACAG294450.014.7632715
ACTGTGC249600.014.3486138
GTATTAG234700.014.297551
TTATACT370950.014.2290524
CCTATAC255500.014.1146163
GTATATA613300.013.9963511
TATACCG171850.013.9609485
GTATTAT337450.013.8456731
ACAGTGC278300.013.8417438
GTATAGA264950.013.7056451
CGCCGTA303600.013.69724394-95
TACACTG255050.013.6537435
TCTATAC245650.013.616773
TAGACTG179950.013.4645365
GTATAAG229600.013.3316531
Produced by FastQC (version 0.11.2)