FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n02_100.3520000006b26e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n02_100.3520000006b26e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26708329
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG170950.019.1201865
CTATACT209100.017.3585934
TAGACTG86100.016.8294325
ACTGTGC125500.016.4636928
TACACTG124550.016.4020255
TAATACT255100.016.1280784
TTATACT255300.016.0968344
ATACTGT175450.016.0009236
TAAGGCT110550.015.9867214
TTACACT148450.015.7776364
GTATAGG142650.015.5932391
AGACTGT88350.015.4307236
TAAGACT118350.015.3345234
CTACACT130950.015.2739644
GACTGTG84100.015.1370357
TTAACCT151000.015.1336374
TAGACCT93900.015.12797454
GGACCGT62500.015.1246036
CAGGACT88000.015.0624884
CTACCCT106600.015.0192384