FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n01_87.3510000006b105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n01_87.3510000006b105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46862694
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG291450.018.0233655
CTATACT335350.016.4856824
ACTGTGC216050.015.6740098
TAATACT447050.015.6494434
TTATACT436250.015.4598464
TAGACTG155800.015.1202535
TACACTG215600.014.58324055
TTACACT257650.014.5260764
TAAGACT210150.014.5096824
CTACACT218250.014.4717024
ATACTGT309200.014.4081486
TAAGGCT188950.014.3780974
TATACCG149650.014.3451985
GTATAGG222150.014.3306741
TAGACAG153500.014.2948115
TGGACTG213650.014.071675
CTACCCT174100.014.0222144
CTAGACT173500.013.7422074
TCTATAC268150.013.6921613
CTGTGCA317750.013.60193359