FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n01_86.3510000006b0b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n01_86.3510000006b0b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36132769
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG221550.016.6994255
CTATACT242700.015.4792014
TAGACTG113450.014.5683565
ACTGTGC161450.014.3554748
TTATACT306050.014.3546034
TACACTG169450.014.2102655
TAATACT309100.013.9363844
TATACCG106250.013.320585
TTACACT197550.013.1988824
TATACAG208200.012.91134555
TAGACAG126250.012.7527595
GTATTGG295000.012.6914531
GTATTAC172550.012.66630651
ATACTGT235950.012.580446
TCTATAC187650.012.5796593
TAGGACT151800.012.5775514
CCTATAC150650.012.5164863
GTATAGG154300.012.4708561
GTATTAG178450.012.4339031
CTACACT168350.012.4131334