FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n01_101.3510000006b2e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n01_101.3510000006b2e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57245972
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG311600.017.5131365
GTATAGG248400.016.220631
CTATACT324000.016.0807534
TAGACTG183300.015.6500525
TAATACT361950.015.4838234
TTATACT374100.015.4125944
GTATTGG416800.014.5688941
GTATATA635150.014.5276841
TATACAG301400.014.5130185
TCTATAC249800.014.4125263
TATACCG177400.014.2964755
ACTGTGC248300.014.2884418
GTATTAG241250.014.2591851
GTATAGA260950.013.9656611
CCTATAC245200.013.7918623
GTATTAT341000.013.7805141
TACACTG255950.013.69438655
TAGACAG187250.013.595165
ATTATAC335750.013.5098563
ATAATAC338800.013.1078533