FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l02n01_100.3510000006b261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l02n01_100.3510000006b261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26708329
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG170150.019.2880275
TAGACTG88100.017.143245
CTATACT212450.016.9680394
TTATACT249050.016.8582424
TAATACT254950.016.6171444
TACACTG123150.016.583485
GGACCGT57150.016.1223076
TAGACCT97200.016.0759324
TAGGGCT102550.015.6540824
TAAGGCT114900.015.6249394
CTACCCT102100.015.5370054
TTACACT150350.015.5104594
TAAGACT116400.015.3419814
TAGACAG91800.015.21065
TTAACCT152350.015.1197924
ACTGTGC126650.014.962688
CAGAGGT164150.014.9587824
CTACACT125700.014.8870124
GTATTGG190600.014.8325661
ATACTGT178900.014.8137676