FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l01n02_68.3520000006b2f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l01n02_68.3520000006b2f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30523711
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG203750.022.0633745
CTATACT222450.020.3582764
TAATACT262200.019.1205464
CCTATAC164300.018.6847323
TTATACT256350.018.6300184
ATAATAC236250.017.721323
GTATAGG173650.017.3863331
GTATTAT238200.017.3255521
TATACAG188800.017.3166895
TAGACTG111250.017.2994945
TCTATAC168650.016.7657343
ATTATAC233550.016.6239453
ACTGTGC149800.016.1711568
GTATAGA174500.015.8848171
GTATTAG150850.015.8222231
GTATATA480450.015.7841641
TATACCG104800.015.77961355
GTATTGG200150.015.7021
ATACTGT219100.015.6559466
TACACTG144800.015.5556525