FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l01n02_66.3520000006b1e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l01n02_66.3520000006b1e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28750824
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG204500.022.9356565
CTATACT252450.019.2381654
TACACTG140050.018.7981035
TAGACTG92300.018.6892685
ATACTGT200550.018.5253816
ACTGTGC139400.017.3434458
TTATACT322900.017.1600534
CCTATAC148750.016.9643123
TAATACT331800.016.9289174
GTATTGG239150.016.4617061
TATACAG184700.016.3378245
GTATAGG153850.015.9774671
TACACAG130700.015.816165
CTGTGCG68800.015.8092049
ACTGTGT157250.015.797618
CTACACT141000.015.7393174
TACTGTG153650.015.7373937
TAGGACT119650.015.7278964
GACTGTG85300.015.5382757
TCTATAC193650.015.4358963