FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l01n02_65.3520000006b16f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l01n02_65.3520000006b16f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20041971
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG145800.023.3363825
TACACTG103350.022.6680665
CTACACT101500.021.16184
ACTGTGC96750.020.4723158
CTATACT161350.020.056654
GGACTGT60600.019.7566266
CTGTGCG54900.019.4657659
TTATACT224850.018.894044
TTACACT129000.018.8239784
TAGGCTG96950.018.7237835
TAGACTG65200.018.6582285
ATACTGT148550.018.5818236
TAAGGCT86900.018.373834
TAAGACT97550.017.975434
TGTACTG158400.017.9700665
TAATACT233350.017.9614354
TCTATAC134450.017.919923
CTGTGCA138700.017.9096269
TAGAGGT97150.017.7559554
CAGAGGT112850.017.6438224
Produced by FastQC (version 0.11.2)