FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l01n02_61.3520000006b097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l01n02_61.3520000006b097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5016259
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT46050.024.1475874
TAGACTG16700.023.0492085
CCTATAC36300.021.077743
GTATAGG32550.020.5963171
TATACTG32500.020.0320175
TATACAG28050.018.4663525
ATACTGT34050.018.417916
GTGTAGG20450.018.367721
CTGTGCG13550.018.227659
ACTGTGC24700.017.692628
AGACTGT15600.017.6639146
CCCTATA34000.017.6187082
GTACAGG17700.017.4606951
TACACTG24450.017.298115
TACACAG21550.017.2002535
ACTGTGT26600.017.143168
CCTACAC21000.016.9725363
TAAGGCT18950.016.8016934
CTACACT25650.016.6741094
TCTATAC30650.016.5904483