FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l01n02_59.3520000006b047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l01n02_59.3520000006b047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8859090
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG53750.019.9800855
TAGACTG28250.019.6804375
GTATAGG50700.019.2234611
TAGACAG27900.018.0538165
CTATACT65900.017.954934
TACACTG41150.017.7835395
ACTGTGC38250.017.5091978
CCTATAC45850.017.3085883
TATACCG26100.016.932065
GTGTAGG33450.016.3450931
TTATACT83250.015.8112184
ACAGTCC26550.015.7433338
CTAGACT35150.015.5468454
TAATACT82900.015.3620824
TATACAG50500.015.3378175
GGACCGT17700.015.2999656
ATACTGT58650.015.2292566
ACTGCGT17550.015.156178
ACTGTCC27550.014.9994798
GTGTTAG37550.014.9402451