FastQCFastQC Report
Mon 24 Oct 2016
C98UFACXX_l01n01_65.3510000006b162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l01n01_65.3510000006b162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20041971
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG146750.022.6202565
TAGACTG65900.021.6189375
ACTGTGC99800.021.2704968
TACACTG103500.020.7394185
CTATACT162500.020.3133684
CTACCCT80600.020.035234
CTGTGCG54900.019.4630459
TAGACCT75800.019.3615324
TAGAGGT101900.019.1565884
ATACTGT150850.018.6997746
TAATACT232050.018.6460694
TTACACT130800.018.5551154
CAGAGGT113150.018.5531734
TAGGCTG93500.018.538725
TAAGACT101350.018.463884
TACCCTG70500.018.4569515
CTACACT98150.018.3883934
CTAGGCT69550.018.3698524
TTATACT221150.018.169124
GAGGTGT114850.017.8627366