FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l08n01_nutrinetat_vega72_tsu-0_r2o_hn.3510000008b819.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l08n01_nutrinetat_vega72_tsu-0_r2o_hn.3510000008b819.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19801157
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1372140.6929595073661605TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG271110.13691624181354653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC161800.040.3861445
ACGTCTG168300.039.85051715
GCACACG168600.039.8329911
CGTATGC171150.038.16725544
TCGTATG171400.038.15097443
CTCGTAT172850.037.86750442
CACACGT178250.037.68915612
AGTCACG172950.037.66042328
AGCACAC182000.037.06102810
ACACGTC182750.036.7980413
GTCACGA178300.036.530429
CACGTCT186750.035.9616714
CGATCAG182150.035.64747233
TCACGAT187750.034.81190530
GAGCACA197600.034.294919
ACGATCA190950.034.09890732
CGTCTGA198700.033.99139416
TCTCGTA192750.033.9673241
TCGGAAG200550.033.657183
CAGTCAC199100.032.88348827
Produced by FastQC (version 0.11.2)