FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l08n01_nutrinetat_vega70_stw-0_r2o_hn.3510000008b791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l08n01_nutrinetat_vega70_stw-0_r2o_hn.3510000008b791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19773389
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC3118931.5773370968426303TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC339300.042.62256245
CGTATGC345050.041.8742244
ACGTCTG349650.041.8184315
GCACACG351000.041.7345111
TCGTATG349850.041.37024343
AGCACAC357150.041.02855710
CACACGT360200.040.67479712
CTCGTAT358500.040.36352542
ACACGTC362650.040.30694613
TCGGAAG371050.039.6037143
CACGTCT369850.039.5466114
GAGCACA374650.039.262839
CGTCTGA377300.038.8611516
TCTCGTA374150.038.6721641
ATCGGAA382100.038.4990582
CAGTCAC378350.038.3786727
GATCGGA383450.038.356871
AATCTCG379200.038.0740839
AGAGCAC386450.038.041838
CTGAACT389500.037.63815719
Produced by FastQC (version 0.11.2)