FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l08n01_nutrinetat_vega68_st-0_r2o_hn.3510000008b76a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l08n01_nutrinetat_vega68_st-0_r2o_hn.3510000008b76a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21185922
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC4411842.0824394614499195TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG228010.10762335479192267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC467450.042.93901445
CGTATGC472400.042.49981344
ACGTCTG481600.042.47434215
GCACACG486200.042.12801411
TCGTATG482350.041.69766643
CACGTCT494400.041.39288714
ACACGTC494750.041.35905513
CTCGTAT486500.041.30204442
AGCACAC497700.041.27222410
CACACGT496550.041.2408512
TCGGAAG506100.040.6208083
GAGCACA510150.040.362979
CGTCTGA508000.040.2448716
TCTCGTA503150.039.96320341
GATCGGA515850.039.8867231
CAGTCAC506000.039.87482527
ATCGGAA519200.039.573292
CATCTCG507700.039.54744739
AGAGCAC525650.039.147838
CGGAAGA527150.039.0458074
Produced by FastQC (version 0.11.2)