FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l08n01_nutrinetat_vega66_shahdara_r2o_hn.3510000008b6e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l08n01_nutrinetat_vega66_shahdara_r2o_hn.3510000008b6e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17959423
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC615970.34297872487328795TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC71900.038.17562529
TCACGCC75000.036.56861530
GTATGCC76800.036.00901445
CACGCCA78400.034.98273531
ACGTCTG80400.034.81114615
CGTATGC79700.034.50161444
GCACACG85150.032.86924411
TCGTATG84200.032.7109743
AGTCACG84950.032.3375828
CGCCAAT85400.032.1416433
TATCTCG86600.031.6178839
ACACGTC90750.030.9649113
ACGCCAA88800.030.88565632
CTCGTAT91050.030.27321242
CACACGT93900.029.7584312
AGCACAC96700.029.12966510
GAGCACA97800.028.8485249
CGTCTGA99850.028.23299816
CACGTCT100800.027.85531414
TCGGAAG106950.026.4108663
Produced by FastQC (version 0.11.2)