FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l08n01_nutrinetat_vega64_sakata_r2o_hn.3510000008b66b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l08n01_nutrinetat_vega64_sakata_r2o_hn.3510000008b66b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16942022
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1042430.615292554808393TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC118250.039.82789245
CGTATGC120900.038.91906444
ACGTCTG127000.037.87545415
GCACACG129850.037.16543611
ACACGTC131500.036.64777413
TCGTATG129500.036.3863943
CACACGT135950.035.59713712
CTCGTAT132950.035.28797542
CGTCTGA139650.034.60566716
CACAGTG135600.034.56159233
AGCACAC140350.034.54528410
CACGTCT141750.033.9501414
CACACAG139450.033.81714231
GAGCACA146800.033.3347639
ACACAGT141450.033.24355732
CAGTCAC145550.032.5845927
TCGGAAG152200.032.0302623
TCTCGTA148950.031.64556141
GATCGGA154600.031.5319121
AGAGCAC155750.031.2754048
Produced by FastQC (version 0.11.2)