FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l08n01_nutrinetat_vega62_oy-0_r2o_hn.3510000008b60e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l08n01_nutrinetat_vega62_oy-0_r2o_hn.3510000008b60e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5559335
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC401810.7227663020846917TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG59490.10700920164012423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG48500.040.12642715
GTATGCC47500.040.1236745
CGTATGC48600.039.26287544
GCACACG50250.039.04240411
ACTGACC49950.038.2419532
TCGTATG50450.038.0015243
ACACGTC52000.037.6419413
CACACGT52200.037.5408212
AGCACAC52800.037.3272810
CTCGTAT51200.037.26671642
AGTCACT51650.036.85158528
CACTGAC52250.036.60163531
CACGTCT54000.036.2061314
GTCACTG54300.035.0531229
TCGGAAG55950.035.0369723
CAGTCAC55200.034.7261527
TCTCGTA55900.034.17147441
GAGCACA57750.034.128419
CGTCTGA57600.033.8260716
AATCTCG58150.033.12011739
Produced by FastQC (version 0.11.2)