FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l08n01_nutrinetat_vega60_n13_r2o_hn.3510000008b586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l08n01_nutrinetat_vega60_n13_r2o_hn.3510000008b586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22055317
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC589520.2672915560451931TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC83600.034.69610645
CGTATGC85850.033.57776644
ACGTCTG88500.032.94686515
GCACACG90000.032.49774611
ACTTAGG93350.030.94638632
TCGTATG97300.029.78804843
CTCGTAT97500.029.65582342
CACACGT99500.029.41755512
CACTTAG100750.028.74039631
TTAGGCA100850.028.71189934
ACACGTC101950.028.6223413
TAGGCAT101050.028.5214835
AGCACAC104600.028.37053110
TCACTTA104000.027.88552730
CTTAGGC107350.027.12011133
AGTCACT110050.026.78142228
CACGTCT113400.025.7720214
GAGCACA117500.025.754239
CGTCTGA116450.025.30953616
TCGGAAG116550.025.2786033
Produced by FastQC (version 0.11.2)