FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l08n01_nutrinetat_vega58_mt-0_r2o_hn.3510000008b536.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l08n01_nutrinetat_vega58_mt-0_r2o_hn.3510000008b536.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22060299
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC4478852.030276198885609TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC486450.042.79370545
ACGTCTG495250.042.41211715
CGTATGC493100.042.17627744
GCACACG502650.041.9936211
ACACGTC502200.041.8968513
CCGATGT498900.041.7464233
TCGTATG499500.041.65371743
TATCTCG500700.041.49289739
CACACGT508400.041.44787212
CTCGTAT503000.041.3521242
AGCACAC516650.040.9472410
CACGTCT515300.040.7880914
GATGTAT512550.040.61269835
TCGGAAG524200.040.428673
ACCGATG517050.040.4158932
GAGCACA524750.040.376129
CGTCTGA521350.040.297516
CGATGTA517000.040.26748334
GATCGGA529900.040.0012861
TCACCGA524000.039.79825630
Produced by FastQC (version 0.11.2)