FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l08n01_nutrinetat_vega56_mr-0_r2o_hn.3510000008b494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l08n01_nutrinetat_vega56_mr-0_r2o_hn.3510000008b494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22707617
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC644640.2838871203438036TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC86500.035.53394745
CGTATGC91350.033.5250944
ACGTCTG92300.033.37006815
GCACACG99400.031.43917711
TCGTATG98750.031.14941243
ACACGTC102500.030.44443513
CACACGT106700.029.37257412
AGCACAC108150.028.89561810
CTCGTAT106300.028.872342
TCACGAT117100.026.61037835
GAGCACA119750.026.548049
CGTCTGA117100.026.4757116
CACGTCT119900.025.95125214
TCGGAAG121900.025.5909293
CAGTCAC121950.025.0906827
CACGATC126300.024.45825236
TCTCGTA126750.024.44290241
GATCGGA129850.023.9884661
ACGATCT128450.023.9611337
GATCTCG129300.023.90864639
Produced by FastQC (version 0.11.2)