FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l07n01_nutrinetat_vega54_mh-1_r2o_hn.3510000008b5c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l07n01_nutrinetat_vega54_mh-1_r2o_hn.3510000008b5c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18040896
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1912811.0602633039955445TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG341370.18922009195108713No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA234560.13001571540570933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC215250.041.89856745
ACGTCTG226150.041.1080215
CGTATGC219950.040.97256544
GCACACG228200.040.74859211
TCGTATG224600.040.28447743
AGTCACG225550.039.94055628
GTCACGA228000.039.4521629
ACACGTC236400.039.30659513
AGCACAC237450.039.30333710
CACACGT237900.039.14387512
CTCGTAT233850.038.7179542
CACGTCT245450.037.87565214
GAGCACA248050.037.805189
CGTCTGA246100.037.7207616
TCGGAAG249750.037.3600123
TCACGAT244950.036.694630
CGATCAG245400.036.6456533
GATCGGA256550.036.3843841
CAGTCAC250550.036.25160227
ATCGGAA258400.036.083252
Produced by FastQC (version 0.11.2)