FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l07n01_nutrinetat_vega52_kn-0_r2o_hn.3510000008b50f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l07n01_nutrinetat_vega52_kn-0_r2o_hn.3510000008b50f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17651370
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1578300.8941515587741915TruSeq Adapter, Index 8 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG186490.10565185591826583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC174900.041.001745
ACGTCTG181600.040.4883715
CGTATGC177150.040.35414544
GCACACG185000.039.91452411
TCGTATG184450.038.8057343
CTCGTAT186350.038.51743342
ACACGTC191500.038.38348813
CACACGT192500.038.26590712
AGCACAC196250.037.69521710
GAGCACA204850.036.1785969
CACGTCT203250.036.17558714
TCGGAAG205200.035.983423
CGTCTGA205900.035.75370416
GATCGGA207350.035.5425721
TCTCGTA204250.035.3378541
ATCGGAA214600.034.344342
CAGTCAC210800.034.2608827
CTGAACT216350.034.14114419
AGAGCAC220450.033.590688
ACTCCAG220700.033.42744423
Produced by FastQC (version 0.11.2)