FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l07n01_nutrinetat_vega50_ge-0_r2o_hn.3510000008b487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l07n01_nutrinetat_vega50_ge-0_r2o_hn.3510000008b487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21461696
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2605261.2139115193878434TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG293200.041.62151715
GTATGCC286800.041.61445645
GCACACG296000.041.35701411
CGTATGC290850.040.9035644
CACACGT307050.039.8686712
CTCGTAT299450.039.81687542
TCGTATG300500.039.73219743
AGCACAC309000.039.6971710
ACACGTC309550.039.48853713
CACGTCT316000.038.5970814
CGTCTGA320000.038.177916
GAGCACA323600.037.9895679
TCGGAAG326150.037.6409873
TCTCGTA322850.036.99076541
CAGTCAC324150.036.9325627
GATCGGA333050.036.8516461
CATCTCG326350.036.46306239
ATCGGAA338500.036.2410772
AGAGCAC339650.036.223848
CAGATCA330500.035.984734
Produced by FastQC (version 0.11.2)