FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l07n01_nutrinetat_vega48_edi-0_r2o_hn.3510000008b41d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l07n01_nutrinetat_vega48_edi-0_r2o_hn.3510000008b41d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23948504
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC7074042.953854654136225TruSeq Adapter, Index 6 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283150.11823285496246447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC753450.043.53178829
GTATGCC758000.043.27905345
ACGTCTG783450.043.25480715
TCACGCC756350.043.23399430
GCACACG786300.043.166711
CACGCCA759850.043.0555831
CACACGT790650.042.90359512
CGTATGC764550.042.88205344
ACACGTC791600.042.85210813
CGCCAAT766200.042.7662933
AGTCACG770250.042.69331428
AGCACAC795600.042.68473410
ACGCCAA768300.042.5849732
TCGTATG773050.042.44830743
TATCTCG774300.042.4296839
CACGTCT804600.042.1541514
CGTCTGA808850.041.91874716
CTCGTAT784200.041.83883342
GAGCACA814750.041.789169
GATCGGA816150.041.766591