FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l07n01_nutrinetat_vega46_ct-1_r2o_hn.3510000008b3be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l07n01_nutrinetat_vega46_ct-1_r2o_hn.3510000008b3be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17761591
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1936101.0900487461962163TruSeq Adapter, Index 5 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG186360.10492303307738592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC215450.041.2032245
ACGTCTG218900.041.1845715
CGTATGC216700.040.9447844
GCACACG224300.040.1328711
TCGTATG225150.039.46795743
CTCGTAT225200.039.42732642
ACACGTC229600.039.26525513
CACACGT230450.039.0325612
AGCACAC234000.038.62308510
CACGTCT235700.038.26814714
CACACAG231950.038.25634831
CACAGTG231900.038.12876533
GAGCACA241250.037.5929539
CGTCTGA240750.037.2972216
TCGGAAG244150.037.0553863
ACACAGT239900.036.87603832
TCTCGTA244150.036.40941641
CAGTCAC246250.036.29058527
TCACACA246050.036.10062830
GATCGGA253050.035.696441
Produced by FastQC (version 0.11.2)