FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l07n01_nutrinetat_vega44_col-0_r2o_hn.3510000008b37b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l07n01_nutrinetat_vega44_col-0_r2o_hn.3510000008b37b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19667206
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC859930.43724055160656783TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG264450.1344624142341317No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA203420.10343106183969397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC107550.037.49267245
ACGTCTG110950.036.94737215
CGTATGC111300.036.18900744
GCACACG115400.035.73708711
ACTGACC118550.033.89552732
ACACGTC122200.033.7116213
TCGTATG120650.033.4776443
CACACGT123300.033.37437412
CACTGAC121850.033.1437331
CTCGTAT123350.032.90710442
AGCACAC126400.032.73385610
AGTCACT127400.031.80583628
CACGTCT130500.031.4985514
GTCACTG131450.030.75742729
GAGCACA138050.030.2485169
CGTCTGA137150.030.05329916
TCGGAAG138650.029.7526363
TCTCGTA141200.028.80901741
CAGTCAC141400.028.72038727
AGAGCAC148450.028.1624188
Produced by FastQC (version 0.11.2)