FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l07n01_nutrinetat_vega42_bur-0_r2o_hn.3510000008b31e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l07n01_nutrinetat_vega42_bur-0_r2o_hn.3510000008b31e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21801747
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC8747864.012458267679191TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC940600.043.6904745
ACGTCTG965950.043.6866215
GCACACG969450.043.63797411
CACACGT976200.043.29013412
AGCACAC979400.043.20842410
CGTATGC950550.043.19309244
ACACGTC979250.043.13003513
ACTTAGG949500.043.0478132
CACGTCT980350.043.0357414
CACTTAG951650.042.93873631
TCGTATG959600.042.82794643
TAGGCAT954250.042.82174335
TCACTTA955050.042.78587330
GAGCACA991200.042.7462469
CTCGTAT961750.042.7174742
TTAGGCA957450.042.6668734
AGTCACT961300.042.61301428
CGTCTGA991650.042.52945716
CTTAGGC963650.042.4250533
TCGGAAG999750.042.405363