FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l07n01_nutrinetat_vega40_bi-1_r2o_hn.3510000008b2f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l07n01_nutrinetat_vega40_bi-1_r2o_hn.3510000008b2f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18287821
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC539260.2948738398084714TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC75050.034.09058444
GTATGCC75450.033.99932545
ACGTCTG77050.033.98928515
GCACACG80400.032.5730711
CCGATGT80400.031.84549133
TATCTCG80450.031.7699439
ACACGTC83100.031.51473813
TCGTATG83350.030.93856243
CTCGTAT84150.030.42934642
CACACGT87500.029.98142212
AGCACAC92550.028.66151210
CACGTCT95700.027.29493114
GATGTAT94900.027.24053235
ACCGATG95400.026.93267332
CGATGTA95750.026.85772134
TCGGAAG98300.026.6948763
GAGCACA102300.026.105799
GATCGGA102250.025.7058931
CACCGAT100800.025.60145231
TCTCGTA101550.025.4125141
Produced by FastQC (version 0.11.2)